Plasmid_Backbone

Part:BBa_K4294093:Design

Designed by: Moore et al, Paul Freemont lab   Group: iGEM22_Athens   (2022-10-12)


pTU2-A-RFP (p15A origin)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 107
    Plasmid lacks a suffix.
    Illegal XbaI site found at 140
    Illegal XbaI site found at 150
    Illegal PstI site found at 1259
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 107
    Illegal PstI site found at 1259
    Illegal NotI site found at 113
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 107
    Illegal XhoI site found at 2231
    Illegal XhoI site found at 3123
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 107
    Plasmid lacks a suffix.
    Illegal XbaI site found at 140
    Illegal XbaI site found at 150
    Illegal PstI site found at 1259
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 107
    Plasmid lacks a suffix.
    Illegal XbaI site found at 122
    Illegal XbaI site found at 140
    Illegal XbaI site found at 150
    Illegal PstI site found at 1259
    Illegal AgeI site found at 942
    Illegal AgeI site found at 1054
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 128
    Illegal BsaI.rc site found at 1253


Design Notes

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Source

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References